Appearances: Batman The Movie (1966), Batman (1989), The Dark Knight (2008), Suicide Squad (2016)
Creators: Jerry Robinson, Bill Finger, Bob Kane
Performers: Cesar Romero, Mark Hamill (voice), Jack Nicholson, Heath Ledger, Jared Leto
Defining moment: Most votes specified Ledger’s Oscar-winning take, so we’ll go with his pencil-disappearing “magic trick”. Nasty and deeply unpredictable.
Fascinating fact: Ledger’s Joker never looks at his victims while killing them.